Document Details

Document Type : Article In Journal 
Document Title :
Enhancement of pathologist's routine practice: reuse of DNA extracted from immunostained formalin-fixed paraffin-embedded (FFPE) slides in downstream molecular analysis of cancer
Enhancement of pathologist's routine practice: reuse of DNA extracted from immunostained formalin-fixed paraffin-embedded (FFPE) slides in downstream molecular analysis of cancer
 
Document Language : English 
Abstract : Background/Aim: To date, the conventional formalin-fixed, paraffin-embedded (FFPE) technique is the gold-standard for preserving histomorphology. Once FFPE tissues are stained, slides are routinely archived along with their blocks at biobanks/hospitals. However, the reuse of fixed and stained biospecimens as DNA source is not a common routine practice worldwide and, thus, indicates the need of studies to investigate the feasibility of extracting DNA from already immunohistochemistry (IHC) FFPEstained slides and its possible reuse in subsequent downstream molecular analyses. Materials and Methods: FFPE IHC slides from colorectal cancer (CRC) patients were prepared and stored in the CEGMR Biobank. The workflow consists of digitalization of IHC stained slide’s image, removing the slide cover-slip, crude dissection and DNA extraction. Following DNA quality assessment, mutation analysis of CTNNB1 and methylation profile of CDH1 were performed. Results: High-quality DNA was obtained allowing 60% concordance between CDH1 methylation and membranous E-cadherin expression pattern. Clean CTNNB1 DNA chromatograms with evenly-spaced peaks were observed. Conclusion: This study is a proof of concept to recycle and reuse DNA from IHC stained slides with suitable concentration and integrity for further downstream molecular applications. These findings will enhance the pathologists’ knowledge, attitudes and practices (KAP) towards the use of these biospecimens and support the implementation of this approach in clinical pathology practice. Therefore, the scientific community will benefit from the largest comprehensive database of human fully annotated FFPE biospecimens already available at their disposal in order to demystify the complexity and the heterogeneity of many challenging diseases and foster the transition towards precision medicine. 
ISSN : 1109-6535 
Journal Name : Cancer Genomics-Proteomics 
Volume : 13 
Issue Number : 5 
Publishing Year : 1437 AH
2016 AD
 
Article Type : Article 
Added Date : Wednesday, July 12, 2017 

Researchers

Researcher Name (Arabic)Researcher Name (English)Researcher TypeDr GradeEmail
ASMAA AL-ATTASAL-ATTAS, ASMAA ResearcherDoctorate 
MOURAD ASSIDIASSIDI, MOURAD ResearcherDoctorate 
JAUDAH AL-MAGHRABIAL-MAGHRABI, JAUDAH ResearcherDoctorate 
ASHRAF DALLOLDALLOL, ASHRAF ResearcherDoctorate 
ADEL ABUZENADAHABUZENADAH, ADEL ResearcherDoctorate 
BRUCE BUDOWLEBUDOWLE, BRUCE ResearcherDoctorate 
HANS-JUERGEN SCHULTENSCHULTEN, HANS-JUERGEN ResearcherDoctorate 
MUHAMMAD ABU-ELMAGDABU-ELMAGD, MUHAMMAD ResearcherDoctorate 
ADEEL CHAUDHARYCHAUDHARY, ADEEL ResearcherDoctorate 
ABDELBASET BUHMEIDABUHMEIDA, ABDELBASET ResearcherDoctorate 
MOHAMMED Al-QahtaniAL-QAHTANI, MOHAMMED ResearcherDoctorate 

Files

File NameTypeDescription
 41984.pdf pdf 

Back To Researches Page